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Beer and Cider Analysis – Example of Spectral Repeatability of Benchtop 60 MHz NMR System

by Beer, Benchtop NMR, Cider, NMR, qNMR Tagged: , , , ,

Here is an example of spectral reproducibility. We are doing a lot of beer NMR at the moment on our 300 MHz NMR and for “giggles” we are running many samples through the various bench-top systems in our lab. We have been quantifying small organic acids (lactic, acetic, succinic , malic, citric, etc.) as they can give some idea of yeast activities and health during fermentation. We are also quantifying  and studying the 1,4/1,6 linkage distribution of residual dextrins. The series of superimposed spectra below consists of 28 spectra of a freeze dried beer sample (a unique Belgian Dubbel. Each spectrum was 128 pulses and took approximately 30 minutes per spectrum. So the superimposed data represents a 14 hour continuous stability test.The data was automatically processed with 16K zero-fill and autophase.It looks pretty damn good.

Zoe Belgian Dubbel - Freeze Dried - 1H qNMR analysis. 128 superimposed spectra representing a 14 hour spectral reprodcibility and stability test.
Zoe Belgian Dubbel – Freeze Dried – 1H qNMR analysis. 128 superimposed spectra representing a 14 hour spectral reprodcibility and stability test.

 

We’ve been looking at a lot of sour beers – here is a home-brewed Flemish Red aged in an oak barrel – note the high lactic and acetic content.

Flemish Red Ale - Freeze dried - 1H NMR
Flemish Red Ale – Freeze dried – 1H NMR

We’ve also been analyzing a lot of hard ciders – commercial and home-brewed varitieties of various styles – very different from one sample to another in the small molecule and sugar chemistry.

Commercial Cider Example - Freeze Dried Sample - 1H NMR Analysis at 60 MHz
Commercial Cider Example – Freeze Dried Sample – 1H NMR Analysis at 60 MHz

 

Dry Basque Cider Example - Freeze Dried Sample - 1H qNMR Analysis
Dry Basque Cider Example – Freeze Dried Sample – 1H qNMR Analysis

 

1H qNMR at 300MHz or 60 MHz can be utilized to identify and quantify small molecule chemistry in fermentations. Below is an example of a quantitative chemistry report on a series of ciders.

Table - 1H qNMR - SMall Molecule and Sugars Analysis
Table – 1H qNMR – SMall Molecule and Sugars Analysis

 

Beer and Cider Analysis is offered with similar quantitative results is offered for $100 per sample in our analytical lab.